Virus:Article Title: Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA Antibody Sigma-Aldrich Cat#: H6908; RRID: AB_260070 Anti-Flag Antibody Sigma-Aldrich Cat#: F1804; RRID: AB_262044 Anti-b-Catenin Abcam Cat#: ab16051; RRID: AB_443301 Non-phospho (Active)-b-Catenin Cell Signaling Technology Cat#: 8814; RRID: AB_11127203 Anti-Gapdh Mouse Monoclonal Antibody Proteintech Cat#: 60004-1-Ig; RRID: AB_2107436 Goat Anti-Rabbit IgG-HRP Absin Bioscience Cat#: abs20002; RRID: AB_2716554 Goat Anti-Mouse IgG-HRP Absin Bioscience Cat#: abs20001; RRID: AB_2716555 Anti-Pdgfra-Biotin Antibody (Clone APA5) eBioscience Cat#: 13-1401-82; RRID: AB_11127180 Anti-CD45-APC (Clone 30-F11) eBioscience Cat#: 17-0451-83; RRID: AB_469393 Anti-Ter119-APC (Clone TER-119) eBioscience Cat#: 17-5921-82; RRID: AB_469473 Anti-Tie2-APC (Clone TEK4) Biolegend Cat#: 124010; RRID: AB_10897106 Anti-SCGF/CLEC11a R&D Systems Cat#: AF3729; RRID: AB_2083418 Anti-beta Galactosidase Antibodies GeneTex Cat#: GTX77365; RRID: AB_379834 Alexa Fluor Plus 555 Donkey Anti-Goat IgG (H+L) Thermo Fisher Scientific Cat#: A32816; RRID: AB_2762839 Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) (H+L) Jackson ImmunoResearch Cat#: 703-545-155; RRID: AB_2340375 Bacterial and Virus Strains Trans5aChemically Competent Cell TRANS Cat#: CD201-01 Chemicals, Peptides, and Recombinant Proteins Anti-Flag M2 Affinity Gel Sigma-Aldrich Cat#: A2220 Anti-HA Affinity Gel Sigma-Aldrich Cat#: E6779 Anti-Biotin Microbeads Miltenyi Biotec Cat#: 130-090-485 M-CSF PeproTech Cat#: 315-02-10ug RANKL R&D Systems Cat#: 462-TEC-010 Calcein Sigma-Aldrich Cat#: C0875-5G BCIP Sigma-Aldrich Cat#: 11383221001 NBT Sigma-Aldrich Cat#: 11383213001 Z-Gly-Pro-7-AMC BACHEM Cat#: 4002518 Ac-Gly-BoroPro MCE Cat#: HY-101801 1-Phenyl-2-Thiourea Sigma-Aldrich Cat#: P7629 Streptavidin-PE/Cy7 Biolegend Cat#: 405206 3 3 Flag Peptide Sigma-Aldrich Cat#: F4799 Protease Inhibitor Cocktails Thermo Fisher Scientific Cat#: 78425 Type I Collagenase Worthington Cat#: LS004197 Dispase II Sigma-Aldrich Cat#: 04942078001 DNase I Sigma-Aldrich Cat#: D4527-200KU Toluidine Blue Sigma-Aldrich Cat#: 89640 Alizarin Red S Sigma-Aldrich Cat#: A5533 Oil Red O solution Sigma-Aldrich Cat#: O1391 Critical Commercial Assays LipofectamineTM 3000 Transfection Reagent Thermo Fisher Scientific Cat#: L3000008 Pierce ECL Western Blotting Substrates Thermo Fisher Scientific Cat#: 32106 Colloidal Blue Staining Kit Invitrogen Cat#: LC6025 (Continued on next page) Cell Reports 33, 108252, October 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acid Phosphatase, Leukocyte (Trap) Kit Sigma-Aldrich Cat#: 387A-1KT MicroVue DPD ELISA Kit Quidel Corporation Cat#: 8007 MicroVue Creatinine Assay Kit Quidel Corporation Cat#: 8009 Rat/Mouse P1NP ELISA Kit Immunodiagnosticsystems Cat#: AC-33F1 5X All-In-One MasterMix Kit ABM Cat#: G492 iTaq Universal SYBR Green Supermix BioRad Cat#: 172-5125 APC BrdU Flow Kit BD Bioscience Cat#: 552598 Caspase-3/7 Green Detection Reagent Thermo Fisher Scientific Cat#: C10423 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#: RG027 RNA Clean Kit TIANGEN Cat#: DP412 MEGAshortscript T7 Transcription Kit Ambion Cat#: AM1354 mMESSAGE mMACHINE SP6 Transcription Kit Ambion Cat#: AM1340 BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit Vectorlab Cat#: SK-5400 ANTI-DIGOXIGENIN-POD, FAB-FRG (150 U) Roche Cat#: 11277073910 Experimental Models: Cell Lines Human: HEK293T ATCC Cat#: CRL-3216 Mouse: MC3T3-E1 (Subclone 4) ATCC Cat#: CRL-2593 Experimental Models: Organisms/Strains Mouse: B6;129P2-Faptm2Schn/J The Jackson Laboratory Cat#: 024288 Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J The Jackson Laboratory Cat#: 013134 Mouse: C57BL/6J The Jackson Laboratory Cat#: 000664 Mouse: Osteolectin / Yue et al., 2016a N/A Zebrafish: Tubingen wild type Hu et al., 2019 N/A Recombinant DNA M50 Super 8x TOPFlash Veeman et al., 2003 RRID: Addgene_12456 pRL-SV40P Chen and Prywes, 1999 RRID: Addgene_27163 pcDNA3-mFap-HA This paper N/A pcDNA3-mOln-Flag This paper N/A pcDNA3-hFap-HA This paper N/A pcDNA3-hOln-Flag This paper N/A pCS2-GFP This paper N/A Deposited Data RNA-seq Data of BMSCs Deposited to Gene Expression This Paper GEO: GSE145473 Software and Algorithms DESeq2 Bioconductor https://bioconductor.org/packages/release/bioc/ html/DESeq2.html ClusterProfiler Bioconductor http://www.bioconductor.org/packages/release/ bioc/html/clusterProfiler.html FlowJo V10.1 FlowJo LLC https://www.flowjo.com/solutions/flowjo GraphPad Prism 7.0 GraphPad https://www.graphpad.com/ Snapgene V2.3.2 Snapgene https://www.snapgene.com GSEA UC San Diego https://www.gsea-msigdb.org/gsea/index.jsp Other LS Columns Miltenyi Biotec Cat#: 130-042-401 e2 Cell Reports 33, 108252, October 13, 2020
Recombinant:Article Title: Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA Antibody Sigma-Aldrich Cat#: H6908; RRID: AB_260070 Anti-Flag Antibody Sigma-Aldrich Cat#: F1804; RRID: AB_262044 Anti-b-Catenin Abcam Cat#: ab16051; RRID: AB_443301 Non-phospho (Active)-b-Catenin Cell Signaling Technology Cat#: 8814; RRID: AB_11127203 Anti-Gapdh Mouse Monoclonal Antibody Proteintech Cat#: 60004-1-Ig; RRID: AB_2107436 Goat Anti-Rabbit IgG-HRP Absin Bioscience Cat#: abs20002; RRID: AB_2716554 Goat Anti-Mouse IgG-HRP Absin Bioscience Cat#: abs20001; RRID: AB_2716555 Anti-Pdgfra-Biotin Antibody (Clone APA5) eBioscience Cat#: 13-1401-82; RRID: AB_11127180 Anti-CD45-APC (Clone 30-F11) eBioscience Cat#: 17-0451-83; RRID: AB_469393 Anti-Ter119-APC (Clone TER-119) eBioscience Cat#: 17-5921-82; RRID: AB_469473 Anti-Tie2-APC (Clone TEK4) Biolegend Cat#: 124010; RRID: AB_10897106 Anti-SCGF/CLEC11a R&D Systems Cat#: AF3729; RRID: AB_2083418 Anti-beta Galactosidase Antibodies GeneTex Cat#: GTX77365; RRID: AB_379834 Alexa Fluor Plus 555 Donkey Anti-Goat IgG (H+L) Thermo Fisher Scientific Cat#: A32816; RRID: AB_2762839 Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) (H+L) Jackson ImmunoResearch Cat#: 703-545-155; RRID: AB_2340375 Bacterial and Virus Strains Trans5aChemically Competent Cell TRANS Cat#: CD201-01 Chemicals, Peptides, and Recombinant Proteins Anti-Flag M2 Affinity Gel Sigma-Aldrich Cat#: A2220 Anti-HA Affinity Gel Sigma-Aldrich Cat#: E6779 Anti-Biotin Microbeads Miltenyi Biotec Cat#: 130-090-485 M-CSF PeproTech Cat#: 315-02-10ug RANKL R&D Systems Cat#: 462-TEC-010 Calcein Sigma-Aldrich Cat#: C0875-5G BCIP Sigma-Aldrich Cat#: 11383221001 NBT Sigma-Aldrich Cat#: 11383213001 Z-Gly-Pro-7-AMC BACHEM Cat#: 4002518 Ac-Gly-BoroPro MCE Cat#: HY-101801 1-Phenyl-2-Thiourea Sigma-Aldrich Cat#: P7629 Streptavidin-PE/Cy7 Biolegend Cat#: 405206 3 3 Flag Peptide Sigma-Aldrich Cat#: F4799 Protease Inhibitor Cocktails Thermo Fisher Scientific Cat#: 78425 Type I Collagenase Worthington Cat#: LS004197 Dispase II Sigma-Aldrich Cat#: 04942078001 DNase I Sigma-Aldrich Cat#: D4527-200KU Toluidine Blue Sigma-Aldrich Cat#: 89640 Alizarin Red S Sigma-Aldrich Cat#: A5533 Oil Red O solution Sigma-Aldrich Cat#: O1391 Critical Commercial Assays LipofectamineTM 3000 Transfection Reagent Thermo Fisher Scientific Cat#: L3000008 Pierce ECL Western Blotting Substrates Thermo Fisher Scientific Cat#: 32106 Colloidal Blue Staining Kit Invitrogen Cat#: LC6025 (Continued on next page) Cell Reports 33, 108252, October 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acid Phosphatase, Leukocyte (Trap) Kit Sigma-Aldrich Cat#: 387A-1KT MicroVue DPD ELISA Kit Quidel Corporation Cat#: 8007 MicroVue Creatinine Assay Kit Quidel Corporation Cat#: 8009 Rat/Mouse P1NP ELISA Kit Immunodiagnosticsystems Cat#: AC-33F1 5X All-In-One MasterMix Kit ABM Cat#: G492 iTaq Universal SYBR Green Supermix BioRad Cat#: 172-5125 APC BrdU Flow Kit BD Bioscience Cat#: 552598 Caspase-3/7 Green Detection Reagent Thermo Fisher Scientific Cat#: C10423 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#: RG027 RNA Clean Kit TIANGEN Cat#: DP412 MEGAshortscript T7 Transcription Kit Ambion Cat#: AM1354 mMESSAGE mMACHINE SP6 Transcription Kit Ambion Cat#: AM1340 BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit Vectorlab Cat#: SK-5400 ANTI-DIGOXIGENIN-POD, FAB-FRG (150 U) Roche Cat#: 11277073910 Experimental Models: Cell Lines Human: HEK293T ATCC Cat#: CRL-3216 Mouse: MC3T3-E1 (Subclone 4) ATCC Cat#: CRL-2593 Experimental Models: Organisms/Strains Mouse: B6;129P2-Faptm2Schn/J The Jackson Laboratory Cat#: 024288 Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J The Jackson Laboratory Cat#: 013134 Mouse: C57BL/6J The Jackson Laboratory Cat#: 000664 Mouse: Osteolectin / Yue et al., 2016a N/A Zebrafish: Tubingen wild type Hu et al., 2019 N/A Recombinant DNA M50 Super 8x TOPFlash Veeman et al., 2003 RRID: Addgene_12456 pRL-SV40P Chen and Prywes, 1999 RRID: Addgene_27163 pcDNA3-mFap-HA This paper N/A pcDNA3-mOln-Flag This paper N/A pcDNA3-hFap-HA This paper N/A pcDNA3-hOln-Flag This paper N/A pCS2-GFP This paper N/A Deposited Data RNA-seq Data of BMSCs Deposited to Gene Expression This Paper GEO: GSE145473 Software and Algorithms DESeq2 Bioconductor https://bioconductor.org/packages/release/bioc/ html/DESeq2.html ClusterProfiler Bioconductor http://www.bioconductor.org/packages/release/ bioc/html/clusterProfiler.html FlowJo V10.1 FlowJo LLC https://www.flowjo.com/solutions/flowjo GraphPad Prism 7.0 GraphPad https://www.graphpad.com/ Snapgene V2.3.2 Snapgene https://www.snapgene.com GSEA UC San Diego https://www.gsea-msigdb.org/gsea/index.jsp Other LS Columns Miltenyi Biotec Cat#: 130-042-401 e2 Cell Reports 33, 108252, October 13, 2020
Protease Inhibitor:Article Title: Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA Antibody Sigma-Aldrich Cat#: H6908; RRID: AB_260070 Anti-Flag Antibody Sigma-Aldrich Cat#: F1804; RRID: AB_262044 Anti-b-Catenin Abcam Cat#: ab16051; RRID: AB_443301 Non-phospho (Active)-b-Catenin Cell Signaling Technology Cat#: 8814; RRID: AB_11127203 Anti-Gapdh Mouse Monoclonal Antibody Proteintech Cat#: 60004-1-Ig; RRID: AB_2107436 Goat Anti-Rabbit IgG-HRP Absin Bioscience Cat#: abs20002; RRID: AB_2716554 Goat Anti-Mouse IgG-HRP Absin Bioscience Cat#: abs20001; RRID: AB_2716555 Anti-Pdgfra-Biotin Antibody (Clone APA5) eBioscience Cat#: 13-1401-82; RRID: AB_11127180 Anti-CD45-APC (Clone 30-F11) eBioscience Cat#: 17-0451-83; RRID: AB_469393 Anti-Ter119-APC (Clone TER-119) eBioscience Cat#: 17-5921-82; RRID: AB_469473 Anti-Tie2-APC (Clone TEK4) Biolegend Cat#: 124010; RRID: AB_10897106 Anti-SCGF/CLEC11a R&D Systems Cat#: AF3729; RRID: AB_2083418 Anti-beta Galactosidase Antibodies GeneTex Cat#: GTX77365; RRID: AB_379834 Alexa Fluor Plus 555 Donkey Anti-Goat IgG (H+L) Thermo Fisher Scientific Cat#: A32816; RRID: AB_2762839 Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) (H+L) Jackson ImmunoResearch Cat#: 703-545-155; RRID: AB_2340375 Bacterial and Virus Strains Trans5aChemically Competent Cell TRANS Cat#: CD201-01 Chemicals, Peptides, and Recombinant Proteins Anti-Flag M2 Affinity Gel Sigma-Aldrich Cat#: A2220 Anti-HA Affinity Gel Sigma-Aldrich Cat#: E6779 Anti-Biotin Microbeads Miltenyi Biotec Cat#: 130-090-485 M-CSF PeproTech Cat#: 315-02-10ug RANKL R&D Systems Cat#: 462-TEC-010 Calcein Sigma-Aldrich Cat#: C0875-5G BCIP Sigma-Aldrich Cat#: 11383221001 NBT Sigma-Aldrich Cat#: 11383213001 Z-Gly-Pro-7-AMC BACHEM Cat#: 4002518 Ac-Gly-BoroPro MCE Cat#: HY-101801 1-Phenyl-2-Thiourea Sigma-Aldrich Cat#: P7629 Streptavidin-PE/Cy7 Biolegend Cat#: 405206 3 3 Flag Peptide Sigma-Aldrich Cat#: F4799 Protease Inhibitor Cocktails Thermo Fisher Scientific Cat#: 78425 Type I Collagenase Worthington Cat#: LS004197 Dispase II Sigma-Aldrich Cat#: 04942078001 DNase I Sigma-Aldrich Cat#: D4527-200KU Toluidine Blue Sigma-Aldrich Cat#: 89640 Alizarin Red S Sigma-Aldrich Cat#: A5533 Oil Red O solution Sigma-Aldrich Cat#: O1391 Critical Commercial Assays LipofectamineTM 3000 Transfection Reagent Thermo Fisher Scientific Cat#: L3000008 Pierce ECL Western Blotting Substrates Thermo Fisher Scientific Cat#: 32106 Colloidal Blue Staining Kit Invitrogen Cat#: LC6025 (Continued on next page) Cell Reports 33, 108252, October 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acid Phosphatase, Leukocyte (Trap) Kit Sigma-Aldrich Cat#: 387A-1KT MicroVue DPD ELISA Kit Quidel Corporation Cat#: 8007 MicroVue Creatinine Assay Kit Quidel Corporation Cat#: 8009 Rat/Mouse P1NP ELISA Kit Immunodiagnosticsystems Cat#: AC-33F1 5X All-In-One MasterMix Kit ABM Cat#: G492 iTaq Universal SYBR Green Supermix BioRad Cat#: 172-5125 APC BrdU Flow Kit BD Bioscience Cat#: 552598 Caspase-3/7 Green Detection Reagent Thermo Fisher Scientific Cat#: C10423 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#: RG027 RNA Clean Kit TIANGEN Cat#: DP412 MEGAshortscript T7 Transcription Kit Ambion Cat#: AM1354 mMESSAGE mMACHINE SP6 Transcription Kit Ambion Cat#: AM1340 BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit Vectorlab Cat#: SK-5400 ANTI-DIGOXIGENIN-POD, FAB-FRG (150 U) Roche Cat#: 11277073910 Experimental Models: Cell Lines Human: HEK293T ATCC Cat#: CRL-3216 Mouse: MC3T3-E1 (Subclone 4) ATCC Cat#: CRL-2593 Experimental Models: Organisms/Strains Mouse: B6;129P2-Faptm2Schn/J The Jackson Laboratory Cat#: 024288 Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J The Jackson Laboratory Cat#: 013134 Mouse: C57BL/6J The Jackson Laboratory Cat#: 000664 Mouse: Osteolectin / Yue et al., 2016a N/A Zebrafish: Tubingen wild type Hu et al., 2019 N/A Recombinant DNA M50 Super 8x TOPFlash Veeman et al., 2003 RRID: Addgene_12456 pRL-SV40P Chen and Prywes, 1999 RRID: Addgene_27163 pcDNA3-mFap-HA This paper N/A pcDNA3-mOln-Flag This paper N/A pcDNA3-hFap-HA This paper N/A pcDNA3-hOln-Flag This paper N/A pCS2-GFP This paper N/A Deposited Data RNA-seq Data of BMSCs Deposited to Gene Expression This Paper GEO: GSE145473 Software and Algorithms DESeq2 Bioconductor https://bioconductor.org/packages/release/bioc/ html/DESeq2.html ClusterProfiler Bioconductor http://www.bioconductor.org/packages/release/ bioc/html/clusterProfiler.html FlowJo V10.1 FlowJo LLC https://www.flowjo.com/solutions/flowjo GraphPad Prism 7.0 GraphPad https://www.graphpad.com/ Snapgene V2.3.2 Snapgene https://www.snapgene.com GSEA UC San Diego https://www.gsea-msigdb.org/gsea/index.jsp Other LS Columns Miltenyi Biotec Cat#: 130-042-401 e2 Cell Reports 33, 108252, October 13, 2020
Transfection:Article Title: Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA Antibody Sigma-Aldrich Cat#: H6908; RRID: AB_260070 Anti-Flag Antibody Sigma-Aldrich Cat#: F1804; RRID: AB_262044 Anti-b-Catenin Abcam Cat#: ab16051; RRID: AB_443301 Non-phospho (Active)-b-Catenin Cell Signaling Technology Cat#: 8814; RRID: AB_11127203 Anti-Gapdh Mouse Monoclonal Antibody Proteintech Cat#: 60004-1-Ig; RRID: AB_2107436 Goat Anti-Rabbit IgG-HRP Absin Bioscience Cat#: abs20002; RRID: AB_2716554 Goat Anti-Mouse IgG-HRP Absin Bioscience Cat#: abs20001; RRID: AB_2716555 Anti-Pdgfra-Biotin Antibody (Clone APA5) eBioscience Cat#: 13-1401-82; RRID: AB_11127180 Anti-CD45-APC (Clone 30-F11) eBioscience Cat#: 17-0451-83; RRID: AB_469393 Anti-Ter119-APC (Clone TER-119) eBioscience Cat#: 17-5921-82; RRID: AB_469473 Anti-Tie2-APC (Clone TEK4) Biolegend Cat#: 124010; RRID: AB_10897106 Anti-SCGF/CLEC11a R&D Systems Cat#: AF3729; RRID: AB_2083418 Anti-beta Galactosidase Antibodies GeneTex Cat#: GTX77365; RRID: AB_379834 Alexa Fluor Plus 555 Donkey Anti-Goat IgG (H+L) Thermo Fisher Scientific Cat#: A32816; RRID: AB_2762839 Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) (H+L) Jackson ImmunoResearch Cat#: 703-545-155; RRID: AB_2340375 Bacterial and Virus Strains Trans5aChemically Competent Cell TRANS Cat#: CD201-01 Chemicals, Peptides, and Recombinant Proteins Anti-Flag M2 Affinity Gel Sigma-Aldrich Cat#: A2220 Anti-HA Affinity Gel Sigma-Aldrich Cat#: E6779 Anti-Biotin Microbeads Miltenyi Biotec Cat#: 130-090-485 M-CSF PeproTech Cat#: 315-02-10ug RANKL R&D Systems Cat#: 462-TEC-010 Calcein Sigma-Aldrich Cat#: C0875-5G BCIP Sigma-Aldrich Cat#: 11383221001 NBT Sigma-Aldrich Cat#: 11383213001 Z-Gly-Pro-7-AMC BACHEM Cat#: 4002518 Ac-Gly-BoroPro MCE Cat#: HY-101801 1-Phenyl-2-Thiourea Sigma-Aldrich Cat#: P7629 Streptavidin-PE/Cy7 Biolegend Cat#: 405206 3 3 Flag Peptide Sigma-Aldrich Cat#: F4799 Protease Inhibitor Cocktails Thermo Fisher Scientific Cat#: 78425 Type I Collagenase Worthington Cat#: LS004197 Dispase II Sigma-Aldrich Cat#: 04942078001 DNase I Sigma-Aldrich Cat#: D4527-200KU Toluidine Blue Sigma-Aldrich Cat#: 89640 Alizarin Red S Sigma-Aldrich Cat#: A5533 Oil Red O solution Sigma-Aldrich Cat#: O1391 Critical Commercial Assays LipofectamineTM 3000 Transfection Reagent Thermo Fisher Scientific Cat#: L3000008 Pierce ECL Western Blotting Substrates Thermo Fisher Scientific Cat#: 32106 Colloidal Blue Staining Kit Invitrogen Cat#: LC6025 (Continued on next page) Cell Reports 33, 108252, October 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acid Phosphatase, Leukocyte (Trap) Kit Sigma-Aldrich Cat#: 387A-1KT MicroVue DPD ELISA Kit Quidel Corporation Cat#: 8007 MicroVue Creatinine Assay Kit Quidel Corporation Cat#: 8009 Rat/Mouse P1NP ELISA Kit Immunodiagnosticsystems Cat#: AC-33F1 5X All-In-One MasterMix Kit ABM Cat#: G492 iTaq Universal SYBR Green Supermix BioRad Cat#: 172-5125 APC BrdU Flow Kit BD Bioscience Cat#: 552598 Caspase-3/7 Green Detection Reagent Thermo Fisher Scientific Cat#: C10423 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#: RG027 RNA Clean Kit TIANGEN Cat#: DP412 MEGAshortscript T7 Transcription Kit Ambion Cat#: AM1354 mMESSAGE mMACHINE SP6 Transcription Kit Ambion Cat#: AM1340 BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit Vectorlab Cat#: SK-5400 ANTI-DIGOXIGENIN-POD, FAB-FRG (150 U) Roche Cat#: 11277073910 Experimental Models: Cell Lines Human: HEK293T ATCC Cat#: CRL-3216 Mouse: MC3T3-E1 (Subclone 4) ATCC Cat#: CRL-2593 Experimental Models: Organisms/Strains Mouse: B6;129P2-Faptm2Schn/J The Jackson Laboratory Cat#: 024288 Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J The Jackson Laboratory Cat#: 013134 Mouse: C57BL/6J The Jackson Laboratory Cat#: 000664 Mouse: Osteolectin / Yue et al., 2016a N/A Zebrafish: Tubingen wild type Hu et al., 2019 N/A Recombinant DNA M50 Super 8x TOPFlash Veeman et al., 2003 RRID: Addgene_12456 pRL-SV40P Chen and Prywes, 1999 RRID: Addgene_27163 pcDNA3-mFap-HA This paper N/A pcDNA3-mOln-Flag This paper N/A pcDNA3-hFap-HA This paper N/A pcDNA3-hOln-Flag This paper N/A pCS2-GFP This paper N/A Deposited Data RNA-seq Data of BMSCs Deposited to Gene Expression This Paper GEO: GSE145473 Software and Algorithms DESeq2 Bioconductor https://bioconductor.org/packages/release/bioc/ html/DESeq2.html ClusterProfiler Bioconductor http://www.bioconductor.org/packages/release/ bioc/html/clusterProfiler.html FlowJo V10.1 FlowJo LLC https://www.flowjo.com/solutions/flowjo GraphPad Prism 7.0 GraphPad https://www.graphpad.com/ Snapgene V2.3.2 Snapgene https://www.snapgene.com GSEA UC San Diego https://www.gsea-msigdb.org/gsea/index.jsp Other LS Columns Miltenyi Biotec Cat#: 130-042-401 e2 Cell Reports 33, 108252, October 13, 2020
Western Blot:Article Title: Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA Antibody Sigma-Aldrich Cat#: H6908; RRID: AB_260070 Anti-Flag Antibody Sigma-Aldrich Cat#: F1804; RRID: AB_262044 Anti-b-Catenin Abcam Cat#: ab16051; RRID: AB_443301 Non-phospho (Active)-b-Catenin Cell Signaling Technology Cat#: 8814; RRID: AB_11127203 Anti-Gapdh Mouse Monoclonal Antibody Proteintech Cat#: 60004-1-Ig; RRID: AB_2107436 Goat Anti-Rabbit IgG-HRP Absin Bioscience Cat#: abs20002; RRID: AB_2716554 Goat Anti-Mouse IgG-HRP Absin Bioscience Cat#: abs20001; RRID: AB_2716555 Anti-Pdgfra-Biotin Antibody (Clone APA5) eBioscience Cat#: 13-1401-82; RRID: AB_11127180 Anti-CD45-APC (Clone 30-F11) eBioscience Cat#: 17-0451-83; RRID: AB_469393 Anti-Ter119-APC (Clone TER-119) eBioscience Cat#: 17-5921-82; RRID: AB_469473 Anti-Tie2-APC (Clone TEK4) Biolegend Cat#: 124010; RRID: AB_10897106 Anti-SCGF/CLEC11a R&D Systems Cat#: AF3729; RRID: AB_2083418 Anti-beta Galactosidase Antibodies GeneTex Cat#: GTX77365; RRID: AB_379834 Alexa Fluor Plus 555 Donkey Anti-Goat IgG (H+L) Thermo Fisher Scientific Cat#: A32816; RRID: AB_2762839 Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) (H+L) Jackson ImmunoResearch Cat#: 703-545-155; RRID: AB_2340375 Bacterial and Virus Strains Trans5aChemically Competent Cell TRANS Cat#: CD201-01 Chemicals, Peptides, and Recombinant Proteins Anti-Flag M2 Affinity Gel Sigma-Aldrich Cat#: A2220 Anti-HA Affinity Gel Sigma-Aldrich Cat#: E6779 Anti-Biotin Microbeads Miltenyi Biotec Cat#: 130-090-485 M-CSF PeproTech Cat#: 315-02-10ug RANKL R&D Systems Cat#: 462-TEC-010 Calcein Sigma-Aldrich Cat#: C0875-5G BCIP Sigma-Aldrich Cat#: 11383221001 NBT Sigma-Aldrich Cat#: 11383213001 Z-Gly-Pro-7-AMC BACHEM Cat#: 4002518 Ac-Gly-BoroPro MCE Cat#: HY-101801 1-Phenyl-2-Thiourea Sigma-Aldrich Cat#: P7629 Streptavidin-PE/Cy7 Biolegend Cat#: 405206 3 3 Flag Peptide Sigma-Aldrich Cat#: F4799 Protease Inhibitor Cocktails Thermo Fisher Scientific Cat#: 78425 Type I Collagenase Worthington Cat#: LS004197 Dispase II Sigma-Aldrich Cat#: 04942078001 DNase I Sigma-Aldrich Cat#: D4527-200KU Toluidine Blue Sigma-Aldrich Cat#: 89640 Alizarin Red S Sigma-Aldrich Cat#: A5533 Oil Red O solution Sigma-Aldrich Cat#: O1391 Critical Commercial Assays LipofectamineTM 3000 Transfection Reagent Thermo Fisher Scientific Cat#: L3000008 Pierce ECL Western Blotting Substrates Thermo Fisher Scientific Cat#: 32106 Colloidal Blue Staining Kit Invitrogen Cat#: LC6025 (Continued on next page) Cell Reports 33, 108252, October 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acid Phosphatase, Leukocyte (Trap) Kit Sigma-Aldrich Cat#: 387A-1KT MicroVue DPD ELISA Kit Quidel Corporation Cat#: 8007 MicroVue Creatinine Assay Kit Quidel Corporation Cat#: 8009 Rat/Mouse P1NP ELISA Kit Immunodiagnosticsystems Cat#: AC-33F1 5X All-In-One MasterMix Kit ABM Cat#: G492 iTaq Universal SYBR Green Supermix BioRad Cat#: 172-5125 APC BrdU Flow Kit BD Bioscience Cat#: 552598 Caspase-3/7 Green Detection Reagent Thermo Fisher Scientific Cat#: C10423 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#: RG027 RNA Clean Kit TIANGEN Cat#: DP412 MEGAshortscript T7 Transcription Kit Ambion Cat#: AM1354 mMESSAGE mMACHINE SP6 Transcription Kit Ambion Cat#: AM1340 BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit Vectorlab Cat#: SK-5400 ANTI-DIGOXIGENIN-POD, FAB-FRG (150 U) Roche Cat#: 11277073910 Experimental Models: Cell Lines Human: HEK293T ATCC Cat#: CRL-3216 Mouse: MC3T3-E1 (Subclone 4) ATCC Cat#: CRL-2593 Experimental Models: Organisms/Strains Mouse: B6;129P2-Faptm2Schn/J The Jackson Laboratory Cat#: 024288 Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J The Jackson Laboratory Cat#: 013134 Mouse: C57BL/6J The Jackson Laboratory Cat#: 000664 Mouse: Osteolectin / Yue et al., 2016a N/A Zebrafish: Tubingen wild type Hu et al., 2019 N/A Recombinant DNA M50 Super 8x TOPFlash Veeman et al., 2003 RRID: Addgene_12456 pRL-SV40P Chen and Prywes, 1999 RRID: Addgene_27163 pcDNA3-mFap-HA This paper N/A pcDNA3-mOln-Flag This paper N/A pcDNA3-hFap-HA This paper N/A pcDNA3-hOln-Flag This paper N/A pCS2-GFP This paper N/A Deposited Data RNA-seq Data of BMSCs Deposited to Gene Expression This Paper GEO: GSE145473 Software and Algorithms DESeq2 Bioconductor https://bioconductor.org/packages/release/bioc/ html/DESeq2.html ClusterProfiler Bioconductor http://www.bioconductor.org/packages/release/ bioc/html/clusterProfiler.html FlowJo V10.1 FlowJo LLC https://www.flowjo.com/solutions/flowjo GraphPad Prism 7.0 GraphPad https://www.graphpad.com/ Snapgene V2.3.2 Snapgene https://www.snapgene.com GSEA UC San Diego https://www.gsea-msigdb.org/gsea/index.jsp Other LS Columns Miltenyi Biotec Cat#: 130-042-401 e2 Cell Reports 33, 108252, October 13, 2020
Staining:Article Title: Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA Antibody Sigma-Aldrich Cat#: H6908; RRID: AB_260070 Anti-Flag Antibody Sigma-Aldrich Cat#: F1804; RRID: AB_262044 Anti-b-Catenin Abcam Cat#: ab16051; RRID: AB_443301 Non-phospho (Active)-b-Catenin Cell Signaling Technology Cat#: 8814; RRID: AB_11127203 Anti-Gapdh Mouse Monoclonal Antibody Proteintech Cat#: 60004-1-Ig; RRID: AB_2107436 Goat Anti-Rabbit IgG-HRP Absin Bioscience Cat#: abs20002; RRID: AB_2716554 Goat Anti-Mouse IgG-HRP Absin Bioscience Cat#: abs20001; RRID: AB_2716555 Anti-Pdgfra-Biotin Antibody (Clone APA5) eBioscience Cat#: 13-1401-82; RRID: AB_11127180 Anti-CD45-APC (Clone 30-F11) eBioscience Cat#: 17-0451-83; RRID: AB_469393 Anti-Ter119-APC (Clone TER-119) eBioscience Cat#: 17-5921-82; RRID: AB_469473 Anti-Tie2-APC (Clone TEK4) Biolegend Cat#: 124010; RRID: AB_10897106 Anti-SCGF/CLEC11a R&D Systems Cat#: AF3729; RRID: AB_2083418 Anti-beta Galactosidase Antibodies GeneTex Cat#: GTX77365; RRID: AB_379834 Alexa Fluor Plus 555 Donkey Anti-Goat IgG (H+L) Thermo Fisher Scientific Cat#: A32816; RRID: AB_2762839 Alexa Fluor 488 Donkey Anti-Chicken IgY (IgG) (H+L) Jackson ImmunoResearch Cat#: 703-545-155; RRID: AB_2340375 Bacterial and Virus Strains Trans5aChemically Competent Cell TRANS Cat#: CD201-01 Chemicals, Peptides, and Recombinant Proteins Anti-Flag M2 Affinity Gel Sigma-Aldrich Cat#: A2220 Anti-HA Affinity Gel Sigma-Aldrich Cat#: E6779 Anti-Biotin Microbeads Miltenyi Biotec Cat#: 130-090-485 M-CSF PeproTech Cat#: 315-02-10ug RANKL R&D Systems Cat#: 462-TEC-010 Calcein Sigma-Aldrich Cat#: C0875-5G BCIP Sigma-Aldrich Cat#: 11383221001 NBT Sigma-Aldrich Cat#: 11383213001 Z-Gly-Pro-7-AMC BACHEM Cat#: 4002518 Ac-Gly-BoroPro MCE Cat#: HY-101801 1-Phenyl-2-Thiourea Sigma-Aldrich Cat#: P7629 Streptavidin-PE/Cy7 Biolegend Cat#: 405206 3 3 Flag Peptide Sigma-Aldrich Cat#: F4799 Protease Inhibitor Cocktails Thermo Fisher Scientific Cat#: 78425 Type I Collagenase Worthington Cat#: LS004197 Dispase II Sigma-Aldrich Cat#: 04942078001 DNase I Sigma-Aldrich Cat#: D4527-200KU Toluidine Blue Sigma-Aldrich Cat#: 89640 Alizarin Red S Sigma-Aldrich Cat#: A5533 Oil Red O solution Sigma-Aldrich Cat#: O1391 Critical Commercial Assays LipofectamineTM 3000 Transfection Reagent Thermo Fisher Scientific Cat#: L3000008 Pierce ECL Western Blotting Substrates Thermo Fisher Scientific Cat#: 32106 Colloidal Blue Staining Kit Invitrogen Cat#: LC6025 (Continued on next page) Cell Reports 33, 108252, October 13, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Acid Phosphatase, Leukocyte (Trap) Kit Sigma-Aldrich Cat#: 387A-1KT MicroVue DPD ELISA Kit Quidel Corporation Cat#: 8007 MicroVue Creatinine Assay Kit Quidel Corporation Cat#: 8009 Rat/Mouse P1NP ELISA Kit Immunodiagnosticsystems Cat#: AC-33F1 5X All-In-One MasterMix Kit ABM Cat#: G492 iTaq Universal SYBR Green Supermix BioRad Cat#: 172-5125 APC BrdU Flow Kit BD Bioscience Cat#: 552598 Caspase-3/7 Green Detection Reagent Thermo Fisher Scientific Cat#: C10423 Dual Luciferase Reporter Gene Assay Kit Beyotime Cat#: RG027 RNA Clean Kit TIANGEN Cat#: DP412 MEGAshortscript T7 Transcription Kit Ambion Cat#: AM1354 mMESSAGE mMACHINE SP6 Transcription Kit Ambion Cat#: AM1340 BCIP/NBT Alkaline Phosphatase (AP) Substrate Kit Vectorlab Cat#: SK-5400 ANTI-DIGOXIGENIN-POD, FAB-FRG (150 U) Roche Cat#: 11277073910 Experimental Models: Cell Lines Human: HEK293T ATCC Cat#: CRL-3216 Mouse: MC3T3-E1 (Subclone 4) ATCC Cat#: CRL-2593 Experimental Models: Organisms/Strains Mouse: B6;129P2-Faptm2Schn/J The Jackson Laboratory Cat#: 024288 Mouse: B6.Cg-Tg(Col1a1*2.3-GFP)1Rowe/J The Jackson Laboratory Cat#: 013134 Mouse: C57BL/6J The Jackson Laboratory Cat#: 000664 Mouse: Osteolectin / Yue et al., 2016a N/A Zebrafish: Tubingen wild type Hu et al., 2019 N/A Recombinant DNA M50 Super 8x TOPFlash Veeman et al., 2003 RRID: Addgene_12456 pRL-SV40P Chen and Prywes, 1999 RRID: Addgene_27163 pcDNA3-mFap-HA This paper N/A pcDNA3-mOln-Flag This paper N/A pcDNA3-hFap-HA This paper N/A pcDNA3-hOln-Flag This paper N/A pCS2-GFP This paper N/A Deposited Data RNA-seq Data of BMSCs Deposited to Gene Expression This Paper GEO: GSE145473 Software and Algorithms DESeq2 Bioconductor https://bioconductor.org/packages/release/bioc/ html/DESeq2.html ClusterProfiler Bioconductor http://www.bioconductor.org/packages/release/ bioc/html/clusterProfiler.html FlowJo V10.1 FlowJo LLC https://www.flowjo.com/solutions/flowjo GraphPad Prism 7.0 GraphPad https://www.graphpad.com/ Snapgene V2.3.2 Snapgene https://www.snapgene.com GSEA UC San Diego https://www.gsea-msigdb.org/gsea/index.jsp Other LS Columns Miltenyi Biotec Cat#: 130-042-401 e2 Cell Reports 33, 108252, October 13, 2020
|
R&D Systems is a verified supplier 
R&D Systems manufactures this product 